Improvement of catalytic performance of endoglucanase CgEndo from Colletotrichum graminicola by site-directed mutagenesis.
Zhou HY, Yi XN, Chen Q, Zhou JB, Li SF, Cai X, Chen DS, Cheng XP, Li M, Wang HY, Chen KQ, Liu ZQ, Zheng YG.
Zhou HY, et al.
Enzyme Microb Technol. 2022 Mar;154:109963. doi: 10.1016/j.enzmictec.2021.109963. Epub 2021 Dec 15.
Enzyme Microb Technol. 2022.
PMID: 34971884
Two mutants, Y63S and N20D/S113T, with 62.31% and 57.14% increased enzyme activities were obtained, respectively. On this basis, their biochemical properties, kinetic parameters, structural information as well as the application in biomass degradation were investigated and …
Two mutants, Y63S and N20D/S113T, with 62.31% and 57.14% increased enzyme activities were obtained, respectively. On this basis, thei …